ABSTRACT
Objective In order to investigate the Mycoplasma pneumonia (MP) infection and drug resistance in children in Fuyang, Hangzhou, and to provide a rational basis for the clinical diagnosis and treatment of MP in children. Methods A total of 1104 respiratory tract samples were collected from children patients with pneumonia. MP and its mutations in 23S rRNA gene locus were detected by real-time PCR. Then analyze pertinence between the clinical data and experimental results were conducted. Results Among 1104 respiratory tract samples, 175 cases (15.85％) were MP positive. Moreover, the mutation of 23S rRNA gene locus existed in 115 MP positive samples with the positive infection rate of 65.71％ . The infection rate (25.74％) and mutation rate (82.69％) in summer (Jul.-Sep.) was higher than other seasons, and the infection rate (11.72％) and mutation rate (40.63％) was the lowest in winter (Jan.-Mar.) . The infection rates of male and female children were 66.67％ and 64.56％, and the mutation rates of male and female children were 66.67％ and 64.56％. Conclusion MP infection may be related with climate and age. 23S rRNA gene locus mutation has a higher detection rate, prompting that MP shows high resistant rate to macrolides.